LINEAGE SWITCH OF A MOUSE PRE-B-CELL LINE (SPGM-1) TO MACROPHAGE-LIKE CELLS AFTER INCUBATION WITH PHORBOL ESTER AND CALCIUM IONOPHORE
- Author(s)
- SPENCKER, T; Neumann, D; Strasser, A; Resch, K; Martin, M;
- Details
- Publication Year 1995-11-13,Volume 216,Issue #2,Page 540-548
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Publication Type
- Journal Article
- Abstract
- The mouse CD5 positive pre-B cell line SPGM-1 can be induced to switch its lineage commitment towards macrophage differentiation by treatment with a combination of phorbol ester and a calcium ionophore. When cultured with these reagents the pre-B cells ceased to proliferate and rapidly became adherent to plastic surfaces. This morphological change was accompanied by the loss of pre-B cell-specific surface markers, such as PB76 and most prominantly the mu heavy chain of the immunoglobulin receptor complex. In addition, the mRNA of the surrogate light chain lambda(5) disappeared while the induction of lysozyme mRNA could be detected. Differentiated SPGM-1 cells phagocytosed latex beads and showed nonspecific esterase activity. The high efficiency and speed of differentiation in this cellular system makes SPGM-1 a highly suitable model for studying the phenomenon of lineage switching during hemopoesis. (C) 1995 Academic Press, Inc.
- Publisher
- ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
- Keywords
- C-FMS; EXPRESSION; LYMPHOCYTES; INVITRO; GENE
- Publisher's Version
- https://doi.org/10.1006/bbrc.1995.2656
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-11-13 12:00:00