Molecular cloning of two novel transmembrane ligands for eph-related kinases (LERKS) that are related to LERK-2
Publication Year 1996, Volume 13, Issue #1-2, Page 141-149
- Journal Title
- GROWTH FACTORS
- Publication Type
- Journal Article
- A search of the nucleic acid database of expressed sequence rags (ESTs) revealed several partial cDNA sequences that could encode proteins homologous to the ligands for Eph-related kinases (LERKs). Oligonucleotides designed from the ESTs were used to probe a human brain cDNA library and obtain overlapping clones that encoded two different novel LERKS (NLERK-1 and NLERK-2). NLERK-1 and NLERK-2 are most closely related to human LERK-2/Elk-ligand and they form a subclass of LERKs that contain a transmembrane domain and a conserved cytoplasmic domain. Full-length NLERK-1 was expressed as a glycosylated membrane protein in COS cells and was not secreted into the medium. Full-length NLERK-2 was similarly expressed in COS cells but both membrane-bound and a truncated, proteolytically- released form were detected. Engineered forms of both NLERK-1 and NLERK-2 lacking transmembrane and cytoplasmic domains were also expressed in COS cells and each was detected in the extracellular medium.
- HARWOOD ACAD PUBL GMBH
- Publisher's Version
- Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-01-01 12:00:00Last Modified: 0001-01-01 12:00:00