Molecular cloning of two novel transmembrane ligands for eph-related kinases (LERKS) that are related to LERK-2
Details
Publication Year 1996, Volume 13, Issue #1-2, Page 141-149
Journal Title
GROWTH FACTORS
Publication Type
Journal Article
Abstract
A search of the nucleic acid database of expressed sequence rags (ESTs) revealed several partial cDNA sequences that could encode proteins homologous to the ligands for Eph-related kinases (LERKs). Oligonucleotides designed from the ESTs were used to probe a human brain cDNA library and obtain overlapping clones that encoded two different novel LERKS (NLERK-1 and NLERK-2). NLERK-1 and NLERK-2 are most closely related to human LERK-2/Elk-ligand and they form a subclass of LERKs that contain a transmembrane domain and a conserved cytoplasmic domain. Full-length NLERK-1 was expressed as a glycosylated membrane protein in COS cells and was not secreted into the medium. Full-length NLERK-2 was similarly expressed in COS cells but both membrane-bound and a truncated, proteolytically- released form were detected. Engineered forms of both NLERK-1 and NLERK-2 lacking transmembrane and cytoplasmic domains were also expressed in COS cells and each was detected in the extracellular medium.
Publisher
HARWOOD ACAD PUBL GMBH
Keywords
SEQUENCE
Rights Notice
Refer to copyright notice on published article.


Creation Date: 1996-01-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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