Micropreparative high performance liquid chromatography of proteins and peptides: Principles and applications
- Author(s)
- Nice, EC;
- Details
- Publication Year 1996,Volume 40,Issue #3,Page 319-341
- Journal Title
- BIOPOLYMERS
- Publication Type
- Journal Article
- Abstract
- The role of micropreparative high performance liquid chromatography columns for the efficient purification of microgram to nanogram quantities of proteins and peptides in small eluent volumes at high concentration is described. The historical development of the technique with respect to column dimensions and instrumentation is outlined. The potential of this technique to prepare samples for use with highly specific and sensitive analytical techniques (e.g., N-terminal sequence analysis, mass spectrometry, biosensor analysis, biological assay) or for the recovery of low levels of material following chemical modification (e.g., radio iodination, enzymatic semisynthesis, chemical conjugation) is illustrated for a number of proteins including growth factors and their receptors. (C) 1996 John Wiley & Sons, Inc.
- Publisher
- JOHN WILEY & SONS INC
- Keywords
- SURFACE-PLASMON RESONANCE; COLONY-STIMULATING FACTOR; AMINO-ACID-SEQUENCE; EPIDERMAL GROWTH-FACTOR; POLYACRYLAMIDE-GEL ELECTROPHORESIS; TYROSINE-PHOSPHORYLATED PEPTIDES; ELECTROSPRAY MASS-SPECTROMETRY; POLYMERASE CHAIN-REACTION; MICROBORE COLUMNS; HIGH-SENSITIVITY
- Publisher's Version
- https://doi.org/10.1002/(SICI)1097-0282(1996)40:3<319::AID-BIP3>3.0.CO;2-Z
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-01-01 12:00:00