Cloning and characterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor
- Author(s)
- Hilton, DJ; Zhang, JG; Metcalf, D; Alexander, WS; Nicola, NA; Willson, TA;
- Details
- Publication Year 1996-01-09,Volume 93,Issue #1,Page 497-501
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Interleukins 4 (IL-4) and 13 (IL-13) have been found previously to share receptor components on some cells, as revealed by receptor cross-competition studies. In the present study, the cloning is described of murine NR4, a previously unrecognized receptor identified on the basis of sequence similarity with members of the hemopoietin receptor family. mRNA encoding NR4 was found in a wide range of murine cells and tissues. By using transient expression in COS-7 cells, NR4 was found to encode the IL-13 receptor alpha chain, a low-affinity receptor capable of binding IL-13 but not IL-4 or interleukins 2, -7, -9, or -15, Stable expression of the IL-13 receptor ac chain (NR4) in CTLL-2 cells resulted in the generation of high-affinity IL-13 receptors capable of transducing a proliferative signal in response to IL-13 and, moreover, led to competitive cross-reactivity in the binding of IL-4 and IL-13, These results suggest that the IL-13 receptor cu chain (NR4) is the primary binding subunit of the IL-13 receptor and may also be a component of IL-4 receptors.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- LEUKEMIA INHIBITORY FACTOR; GAMMA-CHAIN; FUNCTIONAL COMPONENT; IL-2 RECEPTOR
- Publisher's Version
- https://doi.org/10.1073/pnas.93.1.497
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-01-09 12:00:00