NONCYCLING STATE OF PERIPHERAL-BLOOD PROGENITOR CELLS MOBILIZED BY GRANULOCYTE-COLONY-STIMULATING FACTOR AND OTHER CYTOKINES
- Author(s)
- Roberts, AW; Metcalf, D;
- Details
- Publication Year 1995-08-15,Volume 86,Issue #4,Page 1600-1605
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- Incubation with high doses of tritiated thymidine in vitro was used to determine the percent of progenitor cells in the S phase of the cell cycle. Peripheral brood (PB), bone marrow (BM), and spleen populations from mice injected with granulocyte colony-stimulating factor (G-CSF) at 5 mu g/day for 5 days and BM cells from uninjected littermates were assayed. Although the percentage of progenitor cells in S phase in the marrow (47% +/- 5%) and spleen (52% +/- 9%) was increased significantly in G-CSF-treated mice, only a small proportion of PB progenitor cells (PBPC) were in S phase (7% +/- 4%). In normal human subjects injected with G-CSF at 5 or 10 mu g/ kg/d, the proportions of PB myeloid(-1 +/- 4%) and erythroid (0% +/- 8%) progenitor cells in S phase were very low compared with the proportion of myeloid progenitor cells in S phase in normal BM (34% +/- 10%). Similarly, the large majority of steady-state PBPC and PBPC mobilized by interleukin-3 in combination with either granulocyte-macrophage colony-stimulating factor or G-CSF were also found not to be in S phase. Experiments indicated that the low percentages of PBPC in S phase were not ascribable either to inhibitory elements in the blood or to reduced responsiveness to growth factors. (C) 1995 by The American Society of Hematology.
- Publisher
- W B SAUNDERS CO
- Keywords
- HIGH-DOSE CHEMOTHERAPY; AUTOLOGOUS BONE-MARROW; G-CSF; PLATELET RECOVERY; STEM-CELLS; MACROPHAGE; TRANSPLANTATION; PROLIFERATION; INVIVO; MICE
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Creation Date: 1995-08-15 12:00:00