B1 and B2 cells differ in their potential to switch immunoglobulin isotype
- Author(s)
- Tarlinton, DM; McLean, M; Nossal, GJV;
- Details
- Publication Year 1995-12,Volume 25,Issue #12,Page 3388-3393
- Journal Title
- EUROPEAN JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The ability of purified Bla (Ly-1 B) and B2 cells to switch immunoglobulin isotype was assessed by limiting dilution analysis in two in vitro culture systems. When stimulated in the presence of interleukins-4 and -5 by either lipopolysaccharide or CD40 ligand, the frequency of IgG 1 precursors in the Bla population was at most one third that of IgM precursors. In B2 cells, however, the frequency of IgG1 precursors was up to seven times that of IgM precursors. Bla cells were shown to respond to interleukin-4 by virtue of up-regulating major histocompatibility complex class II expression when exposed to the cytokine, precluding non-responsiveness as a reason for not switching to IgG1. Indeed, interleukin-4 was found to specifically induce transcription of the germ-line IgG1 constant region locus in Bla cells as it did in B2 cells. Collectively these results suggest that the ability of B1 cells to respond to isotype switch commitment factors such as interleukin-4 may be secondary to the production of IgM by these cells.
- Publisher
- WILEY-BLACKWELL
- Keywords
- HEAVY-CHAIN TRANSCRIPTS; ANTIBODY-PRODUCTION; GERMLINE TRANSCRIPTS; INTERFERON-GAMMA; LYMPHOCYTES-B; HUMAN CD5+; INTERLEUKIN-4; INDUCTION; EXPRESSION; SECRETION
- Publisher's Version
- https://doi.org/10.1002/eji.1830251228
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-12-01 12:00:00