Organ culture of fetal pig pancreas for transplantation
Details
Publication Year 1995-12-01, Volume 8, Issue #6, Page 361-368
Journal Title
DIABETES NUTRITION & METABOLISM
Publication Type
Journal Article
Abstract
The isolation of islets of Langerhans from the fetal pancreas in vitro may be an effective way of harvesting tissue for transplantation in insulin-dependent diabetes mellitus. Fetal pancreas has the advantage of also containing many islet precursor cells and their preservation may increase the pool of potentially functional cells that can develop after transplantation. Short-term organ culture of fetal pig pancreatic fragments selectively eliminates non-endocrine cells whereas endocrine cells, and their precursors, are preserved, This paper documents the development of the tissue in vitro under two different culture conditions, Culture in 90%O-2/10%CO2 (''HiO(2)'') was compared to conventional culture (''CC'') in 10%CO2/90% air (similar to 20%O-2), HiO(2) prevents ischaemic central necrosis of the tissue fragments and increases islet yield, but prolonged exposure to 90%O-2 leads to generalised cell. death affecting both exocrine and endocrine components; this cannot be prevented by the addition of the oxygen free radical scavengers, nicotinamide and catalase, The amount of insulin, glucagon and somatostatin containing cells in the fetal pig pancreas appeared not to be affected by the different culture conditions as revealed by immunoperoxidase staining, To determine whether islet precursor cells could be detected in the ducts, in situ hybridisation was used to search for pre-pro-insulin mRNA, This was however, only detectable in maturing or mature beta-cells that already contained insulin detectable by immunoperoxidase cytochemistry but not in the putative immature endocrine precursors in the pancreatic ducts.
Publisher
EDITRICE KURTIS S R L
Keywords
LOW-TEMPERATURE CULTURE; HUMAN-TO-MOUSE; ISLET TRANSPLANTATION; MARKED PROLONGATION; XENOGRAFT SURVIVAL; DIABETIC RATS; NUDE-MICE; OXYGEN; CELLS; IMMUNOGENICITY
Rights Notice
Refer to copyright notice on published article.


Creation Date: 1995-12-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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