The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo

Author(s): Mintern, J; Li, M; Davey, GM; Blanas, E; Kurts, C; Carbone, FR; Heath, WR;
Details: Publication Year 1999-12,Volume 77,Issue #6,Page 539-543
Journal Title: IMMUNOLOGY AND CELL BIOLOGY
Publication Type: Journal Article
Abstract: This report examines the use of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) to determine the site, duration and cell type responsible for antigen presentation in vivo. Evidence that CFSE-labelled T cells can be used to determine where various types of antigens are presented, including auto-antigens, oral antigens and cell-associated foreign antigens, is provided. Using this technique, the length of time antigen is presented after acquisition by APC was measured. Finally, CFSE labelling was used to identify the origin of the APC responsible for different forms of antigen presentation.
Publisher: BLACKWELL SCIENCE ASIA
Keywords: CD8(+) T-CELLS; RESTRICTED CROSS-PRESENTATION; SELF-ANTIGENS; TRANSGENIC MICE; VIRUS-INFECTION; FLOW-CYTOMETRY; INDUCTION; DELETION; HELP; AUTOIMMUNITY
Publisher's Version: https://doi.org/10.1046/j.1440-1711.1999.00868.x
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1999-12-01 12:00:00