Leishmania major proteophosphoglycans exist as membrane-bound and soluble forms and localise to the cell membrane, the flagellar pocket and the lysosome
- Author(s)
- Foth, B; Piani, A; Curtis, JM; Ilg, T; McConville, M; Handman, E;
- Details
- Publication Year 2002-12-19,Volume 32,Issue #14,Page 1701-1708
- Journal Title
- INTERNATIONAL JOURNAL FOR PARASITOLOGY
- Publication Type
- Journal Article
- Abstract
- The Leishimania proteophosphoglycan belongs to a family of heterogeneous polypeptides of unusual composition and structure. Here we demonstrate the presence in the parasite of a membrane-bound hydrophobic form of proteophosphoglycan, in addition to the previously described water-soluble form secreted into the culture medium. Phosphatidylinositol-specific phospholipase C treatment of the hydrophobic form of proteophosphoglycan converted it into a water-soluble form, confirming that it has a functional glycosylphosphatidylinositol-anchor, compatible with it being the product of the proteophosphoglycan] gene. Immunofluorescence, immunoelectron microscopy and surface labelling showed that proteophosphoglycan expression was variable in individual cells but that it was present on the surface of both amastigotes and promastigotes, in the flagellar pocket, in endosomes and in the multi-vesicular tubule which is the newly described lysosome. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- PROTOZOAN PARASITE LEISHMANIA; PROTEINS; LIPOPHOSPHOGLYCAN; PROMASTIGOTES; AMASTIGOTES; CLONING; GENES; SURFACE-ANTIGEN-2; IDENTIFICATION; GLYCOCONJUGATE
- Publisher's Version
- https://doi.org/10.1016/S0020-7519(02)00198-4
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2002-12-19 12:00:00