Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum
Details
Publication Year 2003-01-31, Volume 299, Issue #5607, Page 705-708
Journal Title
SCIENCE
Publication Type
Journal Article
Abstract
Transit peptides mediate protein targeting into plastids and are only poorly understood. We extracted amino acid features from transit peptides that target proteins to the relict plastid (apicoplast) of malaria parasites. Based on these amino acid characteristics, we identified 466 putative apicoplast proteins in the Plasmodium falciparum genome. Altering the specific charge characteristics in a model transit peptide by site-directed mutagenesis severely disrupted organellar targeting in vivo. Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting.
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Keywords
CHLOROPLAST TRANSIT PEPTIDES; LEADER SEQUENCE; MOLECULAR CHAPERONES; TOXOPLASMA-GONDII; GENOME SEQUENCE; SIGNAL PEPTIDES; CLEAVAGE SITES; PROTEIN IMPORT; IN-VIVO; MITOCHONDRIAL
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2003-01-31 12:00:00
Last Modified: 0001-01-01 12:00:00
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