Unique monoclonal antibodies define expression of Fc gamma RI on macrophages and mast cell lines and demonstrate heterogeneity among subcutaneous and other dendritic cells
- Author(s)
- Tan, PS; Gavin, AL; Barnes, N; Sears, DW; Vremec, D; Shortman, K; Amigorena, S; Mottram, PL; Hogarth, PM;
- Details
- Publication Year 2003-03-01,Volume 170,Issue #5,Page 2549-2556
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The mouse FcgammaRI is one of the most fundamentally important FcRs. It participates in different stages of immunity, being a low affinity receptor for T-independent IgG3 and yet a high affinity receptor for IgG2a, the product of a Th1 immune response. However, analysis of this receptor has been difficult due largely to the failure to generate specific Abs to this FcR. We have made use of the polymorphic differences between BALB/c and NOD/Lt mice to generate mAb specific for the FcgammaRI of BALB/c and the majority of in-bred mouse strains. Three different mAb were obtained that detected FcgammaRI encoded by the more common Fcgr1(a) and Fcgr1(b) alleles, and although they identified different epitopes, none inhibited the binding of IgG to FcgammaRI. When bound to FcgammaRI, these mAb induced calcium mobilization upon cross-linking. Several novel observations were made of the cellular distribution of FcgammaRI. Resting and IFN-gamma-induced macrophages expressed FcgammaRI as well as mast cell lines. Both bone marrow-derived and freshly isolated dendritic cells from spleen and lymph nodes expressed FcgammaRI. A class of DC, uniquely found in s.c. lymph nodes, expressed the highest level of FcgammaRI and also high levels of MHC class II, DEC205, CD40, and CD86, with a low level of CD8alpha, corresponding to the phenotype for Langerhans-derived DC, which are highly active in Ag processing. Thus, in addition to any role in effector functions, FcgammaRI on APC may act as a link between innate and adaptive immunities by binding and mediating the uptake of T-independent immune complexes for presentation, thereby assisting in the development of T-dependent immune responses.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- MOLECULAR-CLONING; LYMPH-NODES; RECEPTOR; IGG; MICE; IDENTIFICATION; INDUCTION; ANTIGENS
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Creation Date: 2003-03-01 12:00:00