The guanine nucleotide exchange factor C3G is necessary for the formation of focal adhesions and vascular maturation
- Details
- Publication Year 2003-01,Volume 130,Issue #2,Page 355-367
- Journal Title
- DEVELOPMENT
- Publication Type
- Journal Article
- Abstract
- The Ras signalling pathway has major roles in normal cell function and oncogenesis. C3G is a guanine nucleotide exchange factor for members of the Ras family of GTPases. We generated a mouse strain with a hypomorphic C3G allele. C3G(gt/gt) mutant embryos died of vascular defects around E11.5 due to haemorrhage and vascular integrity defects. Vascular supporting cells did not develop appropriately. C3G-deficient fibroblasts responded to PDGF-BB abnormally, exhibited cell adhesion defects and lacked paxillin and integrin-beta1-positive cell adhesions. In contrast, integrin-beta3-positive cell adhesions formed normally. These results show that C3G is required for (1) vascular myogenesis, (2) the formation of paxillin- and integrin beta1-positive, but not integrin beta3-positive, cell adhesions and (3) normal response to PDGF, necessary for vascular myogenesis.
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Keywords
- HEMATOPOIETIC-CELL ADHESION; SIGNALING PATHWAY; ADAPTER PROTEIN; CYTOPLASMIC DOMAINS; MOUSE EMBRYO; R-RAS; CRK; RAP1; ACTIVATION; MICE
- Publisher's Version
- https://doi.org/10.1242/dev.00217
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2003-01-01 12:00:00