Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines
- Author(s)
- Buehr, M; Nichols, J; Stenhouse, F; Mountford, P; Greenhalgh, CJ; Kantachuvesiri, S; Brooker, G; Mullins, J; Smith, AG;
- Details
- Publication Year 2003-01,Volume 68,Issue #1,Page 222-229
- Journal Title
- BIOLOGY OF REPRODUCTION
- Publication Type
- Journal Article
- Abstract
- The POU transcription factor Oct-4 is essential for the pluripotent character of the mouse inner cell mass (ICM) and derivative embryonic stem (ES) cells. We analyzed the expression of Oct-4 during culture and establishment of cell lines from mouse and rat preimplantation embryos. Oct-4 was rapidly lost in primary outgrowths of the majority of cultured embryos prior to any evidence of morphological differentiation. Oct-4 persisted in only a minority of strain 129 cultures, which can go on to give ES cells. We used transgenic rats in which the dual reporter/ selection marker beta-geo is under control of Oct-4 regulatory elements to investigate the effect of direct selection for Oct-4 expressing cells. Ablation of all cells occurred, consistent with complete downregulation of Oct-4. Without selection, in contrast, continuous cultures of morphologically undifferentiated cells could be derived readily from rat blastocysts and lCMs. However, these cells did not express significant Oct-4 and, although capable of differentiating into extraembryonic cell types, appeared incapable of producing fetal germ layer derivatives. Downregulation of Oct-4 appears to be a limiting factor in attempts to derive pluripotent cell lines from preimplantation embryos.
- Publisher
- SOC STUDY REPRODUCTION
- Keywords
- EMBRYONIC STEM-CELLS; DIFFERENTIATION INHIBITING ACTIVITY; TRANSCRIPTION FACTOR; MOUSE EMBRYO; ES CELLS; MAMMALIAN EMBRYO; ESTABLISHMENT; RAT; EXPRESSION; GERM
- Publisher's Version
- https://doi.org/10.1095/biolreprod.102.006197
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2003-01-01 12:00:00