Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells
- Author(s)
- Dimitriadis, E; Robb, L; Salamonsen, LA;
- Details
- Publication Year 2002-07,Volume 8,Issue #7,Page 636-643
- Journal Title
- MOLECULAR HUMAN REPRODUCTION
- Publication Type
- Journal Article
- Abstract
- Differentiation of endometrial stromal cells into decidual cells is crucial for embryo implantation and placentation. Interleukin (IL)-11 signalling is essential for adequate decidualization in the mouse uterus. We examined the role of IL-11 during progesterone-induced decidualization of human endometrial stromal cells over a 10-12 day period, using prolactin (PRL) production as a decidual marker. These cells produced biologically active IL-11 and expressed IL-11, IL-11Ralpha and PRL mRNA during decidualization. Neutralization of endogenous IL-11 with an anti-human (hu)IL-11 antibody (AB) reduced production of PRL from day 8 and insulin-like growth factor binding protein (IGFBP)-1, another marker of decidualization, from day 10 of culture. Following AB washout, PRL and IGFBP-1 secretion increased. Addition of recombinant (r)huIL-11 (10 or 100 ng/ml) to endometrial stromal cells increased secretion of PRL from day 4 and IGFBP-1 from day 6 compared with progesterone alone. Morphological signs of differentiation accompanied biochemical differentiation in the progesterone-treated cells and were further induced by exogenous rhuIL-11. Our observations demonstrate that human endometrial stromal cells produce biologically active IL-11, which promotes progesterone-induced decidualization. These results suggest that IL-11 has both paracrine and autocrine actions on human endometrial stromal cells and plays an important role in preparing the human endometrium for implantation.
- Publisher
- OXFORD UNIV PRESS
- Keywords
- MESSENGER-RIBONUCLEIC-ACID; MENSTRUAL-CYCLE; IN-VITRO; PROLACTIN PRODUCTION; EXPRESSION; TISSUE; IMPLANTATION; RECEPTOR; RELAXIN; DIFFERENTIATION
- Publisher's Version
- https://doi.org/10.1093/molehr/8.7.636
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2002-07-01 12:00:00