Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cells
Author(s)
Lim, JWE; Bodnar, A;
Details
Publication Year 2002-09,Volume 2,Issue #9,Page 1187-1203
Journal Title
PROTEOMICS
Publication Type
Journal Article
Abstract
Human embryonic stem (ES) cells are pluripotent cells with the potential to differentiate into a variety of cell types, which could be used for cell transplantation therapies as well as drug discovery studies. However, the large-scale culture of undifferentiated human ES cells is currently limited by their dependency on mouse embryonic fibroblast feeder layers. The proteomics approach was employed to characterize the environment that supports the growth of undifferentiated human ES cells and to identify factors critical for their independent growth. Conditioned medium from mouse embryonic fibroblast feeder layers, STO cell line, was concentrated and subjected to analyses by two-dimensional electrophoresis mass spectrometry. In total, 136 unique protein species were identified which included some that are known to participate in cell growth and differentiation, extracellular matrix formation and remodeling, in addition to the unexpected but interesting finding of many nominally intracellular proteins. This approach has thus revealed the complexity of the environment provided by the feeder cells and provides a useful starting point for future studies. Moreover, candidates from the initial list of identified proteins can be further investigated for their effects on the growth and differentiation of human ES cells in a defined culture environment.
Publisher
WILEY-BLACKWELL
Keywords
EPITHELIUM-DERIVED FACTOR; CEREBELLAR GRANULE CELLS; FACTOR BINDING PROTEIN-4; IN-VITRO; HUMAN BLASTOCYSTS; FACTOR PEDF; DIFFERENTIATION; SPARC; PROLIFERATION; INDUCTION
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2002-09-01 12:00:00
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