Most lymphoid organ dendritic cell types are phenotypically and functionally immature
- Author(s)
- Wilson, NS; El-Sukkari, D; Belz, GT; Smith, CM; Steptoe, RJ; Heath, WR; Shortman, K; Villadangos, JA;
- Details
- Publication Year 2003-09-15,Volume 102,Issue #6,Page 2187-2194
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- Dendritic cells (DCs) have been thought to follow a life history, typified by Langerhans cells (LCs), with 2 major developmental stages: an immature stage that captures antigens in the periphery and a mature stage that presents those antigens in the lymphoid organs. However, a systematic assessment of the maturity of lymphoid organ DCs has been lacking. We have analyzed the maturity of the DC types found in the steady state in the spleen, lymph nodes (LNs), and thymus. The DCs that migrate into the iliac, mesenteric, mediastinal, or subcutaneous LNs from peripheral tissues were mature and therefore could not process and present newly encountered antigens. However, all the other DC types were phenotypically and functionally immature: they expressed low levels of surface major histocompatibility complex class 11 (MHC 11) and CD86, accumulated MHC 11 in their endosomes, and could present newly encountered antigens. These immature DCs could 1346 induced to mature by culture in vitro or by Inoculation of inflammatory stimuli in vivo. Therefore, the lymphoid organs contain a large cohort of immature DCs, most likely for the maintenance of peripheral tolerance, which can respond to infections reaching those organs and mature in situ. (C) 2003 by The American Society of Hematology.
- Publisher
- AMER SOC HEMATOLOGY
- Keywords
- CLASS-II COMPLEXES; IN-VIVO; ANTIGEN PRESENTATION; LANGERHANS CELLS; DRAINING LYMPH; MOUSE; EXPRESSION; MATURATION; SPLEEN; NODES
- Publisher's Version
- https://doi.org/10.1182/blood-2003-02-0513
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2003-09-15 12:00:00