Normal thymocyte negative selection in TRAIL-deficient mice
- Author(s)
- Cretney, E; Uldrich, AP; Berzins, SP; Strasser, A; Godfrey, DI; Smyth, MJ;
- Details
- Publication Year 2003-08-04,Volume 198,Issue #3,Page 491-496
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- The molecular basis of thymocyte negative selection, which plays a critical role in establishing and maintaining immunological tolerance, is not yet resolved. In particular, the importance of the death receptor subgroup of the tumor necrosis factor (TNF)-family has been the subject of many investigations, with equivocal results. A recent report suggested that TRAIL was a critical factor in this process, a result that does not fit well with previous studies that excluded a role for the FADD-caspase 8 pathway, which is essential for TRAIL and Fas ligand (FasL) signaling, in negative selection. We have investigated intrathymic negative selection of TRAIL-deficient thymocytes, using four well-established models, including antibody-mediated TCR/CD3 ligation in vitro, stimulation with endogenous superantigen in vitro and in vivo, and treatment with exogenous superantigen in vitro. We were unable to demonstrate a role for TRAIL signaling in any of these models, suggesting that this pathway is not a critical factor for thymocyte negative selection.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- APOPTOSIS-INDUCING LIGAND; T-CELL RECEPTOR; FAMILY MEMBER; AUTOREACTIVE THYMOCYTES; IN-VIVO; DELETION; THYMUS; BCL-2; LYMPHADENOPATHY; HOMEOSTASIS
- Publisher's Version
- https://doi.org/10.1084/jem.20030634
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2003-08-04 12:00:00