Analysis of pfcrt, pfmdr1, dhfr, and DHPS mutations and drug sensitivities in Plasmodium falciparum isolates from patients in Vietnam before and after treatment with artemisinin
- Author(s)
- Ngo, T; Duraisingh, M; Reed, M; Hipgrave, D; Biggs, B; Cowman, AF;
- Details
- Publication Year 2003-03-01,Volume 68,Issue #3,Page 350-356
- Journal Title
- AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
- Publication Type
- Journal Article
- Abstract
- We have analyzed artemisinin sensitivity in Plasmodium falciparum isolates obtained from patients in South Vietnam and show that artemisinin sensitivity does not differ before and after drug treatment. There was an increase in the level of mefloquine resistance in the isolates after drug treatment that was concomitant with a decrease in chloroquine resistance, suggesting that treatment with arternisinin has selected for increased mefloquine resistance. Mutations in the pfmdr1 gene, previously shown to be associated with sensitivity to mefloquine, were selected against. All isolates resistant to chloroquine encoded Thr-76 in the pfcrt gene consistent with an essential role in the mechanism of chloroquine resistance. Mutations in pfmdr1 also were linked to chloroquine resistance. High levels of mutation in dhfr and dhps genes, which have previously been associated with Fansidar resistance, also were found, suggesting that this drug would not be useful for malaria control in this part of Vietnam.
- Publisher
- AMER SOC TROP MED & HYGIENE
- Keywords
- MULTIDRUG-RESISTANCE GENE; PYRIMETHAMINE-SULFADOXINE USE; LEVEL CHLOROQUINE RESISTANCE; THYMIDYLATE SYNTHASE GENE; HUMAN MALARIA PARASITE; DIHYDROFOLATE-REDUCTASE; DIHYDROPTEROATE SYNTHASE; IN-VITRO; ANTIMALARIALS; MEFLOQUINE
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- Refer to copyright notice on published article.
Creation Date: 2003-03-01 12:00:00