Schwann cell apoptosis in the postnatal axotomized sciatic nerve is mediated via NGF through the low-affinity neurotrophin receptor
- Author(s)
- Petratos, S; Butzkueven, H; Shipham, K; Cooper, H; Bucci, T; Reid, K; Lopes, E; Emery, B; Cheema, SS; Kilpatrick, TJ;
- Details
- Publication Year 2003-04-01,Volume 62,Issue #4,Page 398-411
- Journal Title
- JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
- Publication Type
- Journal Article
- Abstract
- Schwann cell death is a developmentally regulated phenomenon and is also induced after peripheral nerve axotomy in neonatal rodents. In this study, we explored whether ligand-induced activation of the low-affinity neurotrophin receptor (p75(NTR)) is responsible for inducing Schwann cell death in vivo. Administration of exogenous nerve growth factor (NGF) to the axotomized nerve site in wild-type animals resulted in a 2.6-fold increase in Schwann cell apoptosis in the distal nerve stumps compared to axotomy alone. No increase in apoptosis, above baseline levels, was seen in p75(NTR)-mutant mice either with or without NGF. When anti-NGF antibodies were administered to the site of the peripheral nerve lesion in wild-type mice there was a reduction in the percentage of Schwann cell apoptosis to levels seen in both the quiescent state and in the axotomized nerves of the p75(NTR)-mutant mice, These results demonstrate that apoptosis of Schwann cells in axotomized peripheral nerve is mediated predominantly through p75(NTR) signaling and initiated via endogenously produced NGF.
- Publisher
- AMER ASSN NEUROPATHOLOGISTS INC
- Keywords
- GROWTH-FACTOR RECEPTOR; LEUKEMIA INHIBITORY FACTOR; FOREBRAIN CHOLINERGIC NEURONS; MESSENGER-RNA; GENE-EXPRESSION; P75 RECEPTOR; PERIPHERAL-NERVES; SENSORY NEURONS; DEATH; REGENERATION
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Creation Date: 2003-04-01 12:00:00