The lymphoid past of mouse plasmacytoid cells and thymic dendritic cells
- Author(s)
- Corcoran, L; Ferrero, I; Vremec, D; Lucas, K; Waithman, J; O'Keeffe, M; Wu, L; Wilson, A; Shortman, K;
- Details
- Publication Year 2003-05-15,Volume 170,Issue #10,Page 4926-4932
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- There has been controversy over the possible lymphoid origin of certain dendritic cell (DC) subtypes. To resolve this issue, DC and plasmacytoid pre-DC isolated from normal mouse tissues were analyzed for transient (mRNA) and permanent (DNA rearrangement) markers of early stages of lymphoid development. About 27% of the DNA of CD8(+) DC from thymus, and 22-35% of the DNA of plasmacytoid pre-DC from spleen and thymus, was found to contain IgH gene D-J rearrangements, compared with 40% for T cells. However, the DC DNA did not contain IgH gene V-D-J rearrangements nor T cell Ag receptor 13 gene D-J rearrangements. The same DC lineage populations containing IgH D-J rearrangements expressed mRNA for CD3 chains, and for pre-Talpha. In contrast, little of the DNA of the conventional DC derived from spleen, lymph nodes, or skin, whether CD8(+) or CD8(-), contained IgH D-J rearrangements and splenic conventional DC expressed very little CD3epsilon or pre-Ta mRNA. Therefore, many plasmacytoid pre-DC and thymic CD8(+) DC have shared early steps of development with the lymphoid lineages, and differ in origin from conventional peripheral DC.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- COMMON MYELOID PROGENITOR; PRECURSOR CELLS; CD8 EXPRESSION; BONE-MARROW; T-CELLS; B-CELLS; GENE REARRANGEMENT; NATURAL-KILLER; MICE; POPULATIONS
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Creation Date: 2003-05-15 12:00:00