The cytoplasmic domain of the Plasmodium falciparum ligand EBA-175 is essential for invasion but not protein trafficking
- Author(s)
- Gilberger, TW; Thompson, JK; Reed, MB; Good, RT; Cowman, AF;
- Details
- Publication Year 2003-07-21,Volume 162,Issue #2,Page 317-327
- Journal Title
- JOURNAL OF CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- The invasion of host cells by the malaria parasite Plasmodium, falciparum requires specific protein-protein interactions between parasite and host receptors and an intracellular translocation machinery to power the process. The transmembrane erythrocyte binding protein-175 (EBA-175) and thrombospondin-related anonymous protein (TRAP) play central roles in this process. EBA-175 binds to glycophorin A on human erythrocytes during the invasion process, linking the parasite to the surface of the host cell. In this report, we show that the cytoplasmic domain of EBA-175 encodes crucial information for its role in merozoite invasion, and that trafficking of this protein is independent of this domain. Further, we show that the cytoplasmic domain of TRAP, a protein that is not expressed in merozoites but is essential for invasion of liver cells by the sporozoite stage, can substitute for the cytoplasmic domain of EBA-175. These results show that the parasite uses the same components of its cellular machinery for invasion regardless of the host cell type and invasive form.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- HOST-CELL INVASION; SPOROZOITE SURFACE PROTEIN-2; ERYTHROCYTE BINDING ANTIGEN; PARASITE TOXOPLASMA-GONDII; MALARIA PARASITES; CIRCUMSPOROZOITE PROTEIN; GLIDING MOTILITY; GLYCOPHORIN-A; RECEPTOR; ORGANELLES
- Publisher's Version
- https://doi.org/10.1083/jcb.200301046
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2003-07-21 12:00:00