SOCS3 negatively regulates IL-6 signaling in vivo

Croker, BA; Krebs, DL; Zhang, JG; Wormald, S; Willson, TA; Stanley, EG; Robb, L; Greenhalgh, CJ; Forster, I; Clausen, BE; Nicola, NA; Metcalf, D; Hilton, DJ; Roberts, AW; Alexander, WS

Author(s): Croker, BA; Krebs, DL; Zhang, JG; Wormald, S; Willson, TA; Stanley, EG; Robb, L; Greenhalgh, CJ; Forster, I; Clausen, BE; Nicola, NA; Metcalf, D; Hilton, DJ; Roberts, AW; Alexander, WS;
Details: Publication Year 2003-06,Volume 4,Issue #6,Page 540-545
Journal Title: NATURE IMMUNOLOGY
Publication Type: Journal Article
Abstract: Members of the suppressor of cytokine signaling (SOCS) family are potentially key physiological negative regulators of interleukin-6 (IL-6) signaling. To examine whether SOCS3 is involved in regulating this signaling, we have used conditional gene targeting to generate mice lacking Socs3 in the liver or in macrophages. We show that Socs3 deficiency results in prolonged activation of signal transducer and activator of transcription 1 (STAT1) and STAT3 after IL-6 stimulation but normal activation of STAT1 after stimulation with interferon-gamma (IFN-gamma). Conversely, IL-6-induced STAT activation is normal in Socs1-deficient cells, whereas STAT1 activation induced by IFN-gamma is prolonged. Microarray analysis shows that the pattern of gene expression induced by IL-6 in Socs3-deficient livers mimics that induced by IFN-gamma. Our data indicate that SOCS3 and SOCS1 have reciprocal functions in IL-6 and IFN-gamma regulation and imply that SOCS3 has a role in preventing IFN-gamma-like responses in cells stimulated by IL-6.
Publisher: NATURE PUBLISHING GROUP
Keywords: MICE LACKING SUPPRESSOR; ACUTE-PHASE RESPONSE; IN-VIVO; INTERLEUKIN-6-DEFICIENT MICE; CYTOKINE SIGNALING-3; GAMMA; STAT3; LIVER; ACTIVATION; EXPRESSION
Publisher's Version: https://doi.org/10.1038/ni931
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2003-06-01 12:00:00