Virus infection expands a biased subset of T cells that bind tetrameric class I peptide complexes
Details
Publication Year 2003-06,Volume 33,Issue #6,Page 1557-1567
Journal Title
EUROPEAN JOURNAL OF IMMUNOLOGY
Publication Type
Journal Article
Abstract
We have used a TCR beta-chain transgenic mouse to examine the relationship between the ability of a T cell to bind soluble class I-peptide complexes and its response to antigenic stimulation in vivo. T cells from gBT-1.3beta TCR beta-chain transgenic mice preferentially carried TCR alpha-chains bearing the same Valpha2 V region as found in the parent receptor specific for an immunodominant HSV-1 gB-peptide. Furthermore, CD8(+) T cells from these mice bound K-b-gB tetrameric complexes with relatively high frequency, and most of these cells contained a Valpha2 TCR alpha-chain. Detailed sequence analysis of the tetramer-binding peripheral T cells showed that this was a heterogenous population expressing TCR with only partial sequence similarity to the parent receptor, which took the form of preferential inclusion of the parental Jalpha16 element. Infection with HSV-1, however, selected a subset of tetramer-positive T cells. This was based on the emergence of a co-dominant Jalpha usage and selection of a restricted CDR3alpha length. Therefore, the ability to bind soluble MHC-peptide complexes does not always correlate with the ability of a T cell to respond to its cognate antigen after in vivo stimulation.
Publisher
WILEY-V C H VERLAG GMBH
Keywords
HERPES-SIMPLEX-VIRUS; NEGATIVE SELECTION; RECEPTOR; TCR; RECOGNITION; ANTIGEN; REPERTOIRE; AVIDITY; LYMPHOCYTES; CTL
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Creation Date: 2003-06-01 12:00:00
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