Gene gun immunization in a preclinical model is enhanced by B7 targeting
- Author(s)
- Tachedjian, M; Boyle, JS; Lew, AM; Horvatic, B; Scheerlinck, JPY; Tennent, JM; Andrew, ME;
- Details
- Publication Year 2003-06-20,Volume 21,Issue #21-22,Page 2900-2905
- Journal Title
- VACCINE
- Publication Type
- Journal Article
- Abstract
- DNA vaccines have great potential but despite the promise shown in rodent models, responses in large animals, including humans, have been disappointing. Furthermore, gene gun delivery of DNA has been used to improve these responses. However, most cells that are transfected are not the professional antigen presenting cells (APC) which are critical for generating the primary immune response. Here, we show that in the large animal model of the pig, the combination of the use of gene gun delivery and a DNA vector that targets antigen presenting cells by expressing a CTLA4-ovalbumin (OVA) fusion antigen, leads to enhanced ovalbumin specific serum IgG, IgA, IgG1 and IgG2 immune responses. (C) 2003 Elsevier Science Ltd. All rights reserved.
- Publisher
- ELSEVIER SCI LTD
- Keywords
- DNA VACCINE; IMMUNE-RESPONSES; DENDRITIC CELLS; ANTIGEN; INDUCTION; ANTIBODY; EFFICACY; FUSION; HUMANS; CTLA-4
- Publisher's Version
- https://doi.org/10.1016/S0264-410X(03)00162-2
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2003-06-20 12:00:00