Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo
- Author(s)
- Li, M; Davey, GM; Sutherland, RM; Kurts, C; Lew, AM; Hirst, C; Carbone, FR; Heath, WR;
- Details
- Publication Year 2001-05-15,Volume 166,Issue #10,Page 6099-6103
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- To better understand the antigenic requirements for cross-presentation, we compared the in vivo efficiency of presentation of cell-associated vs soluble OVA with the OT-I (CD8) and OT-II (CD4) TCR transgenic lines. Cross-presentation of cell-associated OVA was very efficient, requiring as little as 21 ng of OVA to activate OT-II cells and 100-fold less to activate OT-I cells. In contrast, soluble OVA was presented inefficiently, requiring at least 10,000 ng OVA for activation of either T cell subset. Thus, cell-associated OVA was presented 500-fold more efficiently than soluble OVA to CD4 T cells and 50,000-fold more efficiently to CD8 T cells. These data, which represent the first quantitative in vivo analysis of cross-presentation, show that cell-associated OVA is very efficiently presented via the class 1 pathway.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- SELF-ANTIGENS; DENDRITIC CELLS; APOPTOTIC CELLS; IN-VIVO; TOLERANCE; INDUCTION; RESPONSES; DELETION; HELP
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- Refer to copyright notice on published article.
Creation Date: 2001-05-15 12:00:00