Effects of a dominant interfering mutant of FADD on signal transduction in activated T cells
- Author(s)
- Newton, K; Kurts, C; Harris, AW; Strasser, A;
- Details
- Publication Year 2001-02-20,Volume 11,Issue #4,Page 273-276
- Journal Title
- CURRENT BIOLOGY
- Publication Type
- Journal Article
- Abstract
- The cytoplasmic adaptor protein FADD is an essential component of the death-inducing signaling complexes (DISCs) that assemble when TNF receptor family members, such as Fas, are ligated, FADD inititates the proteolytic cascade that leads to apoptosis by binding to and promoting the autocatalytic activation of caspase-8 [1-4], Surprisingly, FADD (but not caspase-8) is also required for T cells to proliferate upon their stimulation with mitogens [5-9]. Using transgenic mice expressing a dominant-negative mutant of FADD (FADD-DN), we show that functional FADD is required for T cells to proliferate in response to antigens in vivo as well as to mitogens in culture. The costimulation of wild-type and FADD-DN T cells with mitogens revealed that FADD-DN T cells have a cell-autonomous defect in intracellular signaling. In contrast to another study [6], p53 deficiency did not rescue mitogen-induced proliferation of FADD-DN T cells, and neither did enforced expression of the apoptosis inhibitor Bcl-2, Like wild-type T cells, FADD-DN T cells stimulated with mitogens mobilized intracellular calcium and activated members of the UP-KS transcription factor family as well as p38 mitogen-activated protein kinase (MAPK) and p44/42 MAPK, Therefore, FADD must act downstream of or in parallel to these signaling pathways.
- Publisher
- CELL PRESS
- Keywords
- INHIBITS PROLIFERATION; MICE LACKING; FADD/MORT1; APOPTOSIS; DEATH; THYMOCYTES; DELETION; LEADS
- Publisher's Version
- https://doi.org/10.1016/S0960-9822(01)00067-7
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-02-20 12:00:00