TNF and CD95 promote IL-8 gene transactivation via independent elements in colon carcinoma cells
- Author(s)
- Verhagen, AM; Pakusch, M; Silke, J; Vaux, DL;
- Details
- Publication Year 2001-07-21,Volume 15,Issue #2,Page 108-112
- Journal Title
- CYTOKINE
- Publication Type
- Journal Article
- Abstract
- The pro-inflammatory cytokine interleukin-8 (IL-8) is produced by HT29 colon epithelial cells following engagement of either CD95 or tumour necrosis factor (TNF) receptors. While the IL-8 promotor elements activated by TNF are well characterised, those responsible for induction of IL-8 by CD95 are unknown. We examined the pathway for CD95 induced IL-8 secretion using two luciferase reporter constructs; the first comprising approximately 500 bp of the IL-8 promotor that includes the nuclear factor kappa B (NF kappaB), C/EBP and AP-1 sites known to be involved in TNF mediated IL-8 induction; the second that encompasses these elements but extends approximately 1.1 kb further upstream. Although IL-8 mRNA and protein were produced in response to either TNF or CD95 ligation, only TNF induced an increase in the reporter activity of the promoter constructs. Nevertheless, IL-8 induction by CD95 resulted primarily from increased transcription and not from an increase in IL-8 mRNA stability. These results suggest that promoter elements/enhancers involved in CD95 mediated IL-8 induction are distinct from those used by TNF and not contained within the 1.6 kb region immediately upstream of the initiation codon. (C) 2001 Academic Press.
- Publisher
- W B SAUNDERS CO
- Keywords
- NF-KAPPA-B; FAS-MEDIATED APOPTOSIS; INTERLEUKIN-8 GENE; ACTIVATION; EXPRESSION; ALPHA; PROTEIN; TRAF2; AP-1
- Publisher's Version
- https://doi.org/10.1006/cyto.2001.0915
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-07-21 12:00:00