Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, Syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in B16 melanoma cells

Wade, N; Bryant, NJ; Connolly, LM; Simpson, RJ; Luzio, JP; Piper, RC; James, DE

Author(s): Wade, N; Bryant, NJ; Connolly, LM; Simpson, RJ; Luzio, JP; Piper, RC; James, DE;
Details: Publication Year 2001-06-08,Volume 276,Issue #23,Page 19820-19827
Journal Title: JOURNAL OF BIOLOGICAL CHEMISTRY
Publication Type: Journal Article
Abstract: Syntaxin 7 is a mammalian target soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) involved in membrane transport between late endosomes and lysosomes. The aim of the present study was to use immunoaffinity techniques to identify proteins that interact with Syntaxin 7. We reasoned that this would be facilitated by the use of cells producing high levels of Syntaxin 7, Screening of a large number of tissues and cell lines revealed that Syntaxin 7 is expressed at very high levels in B16 melanoma cells. Moreover, the expression of Syntaxin 7 increased in these cells as they underwent melanogenesis. From a large scale Syntaxin 7 immunoprecipitation, we have identified six polypeptides using a combination of electrospray mass spectrometry and immunoblotting. These polypeptides corresponded to Syntaxin 7, Syntaxin 6, mouse Vps10p tail interactor 1b (mVti1b), alpha -synaptosome-associated protein (SNAP), vesicle-associated membrane protein (VAMP)8, VAMP7, and the protein phosphatase 1M regulatory subunit. We also observed partial colocalization between Syntaxin 6 and Syntaxin 7, between Syntaxin 6 and mVti1b, but not between Syntaxin 6 and the early endosomal t-SNARE Syntaxin 13. Based on these and data reported previously, we propose that Syntaxin 7/mVti1b/Syntaxin 6 may form discrete SNARE complexes with either VAMP7 or VAMPS to regulate fusion events within the late endosomal pathway and that these events may play a critical role in melanogenesis.
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Keywords: LATE ENDOSOME-LYSOSOME; V-SNARE VTI1P; TRANSPORT PATHWAYS; MYOSIN-V; SUBCELLULAR-LOCALIZATION; VESICULAR TRANSPORT; MOLECULAR-CLONING; PLASMA-MEMBRANE; T-SNARE; FUSION
Publisher's Version: https://doi.org/10.1074/jbc.M010838200
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2001-06-08 12:00:00