Cytotoxic T cells to an epitope in the islet autoantigen IA-2 are not disease-specific
- Author(s)
- Takahashi, K; Honeyman, MC; Harrison, LC;
- Details
- Publication Year 2001-06,Volume 99,Issue #3,Page 360-364
- Journal Title
- CLINICAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Cytotoxic CD8 T lymphocytes (CTL) are effecters of pancreatic islet beta -cell destruction in type 1 diabetes but, with the exception of a single report, CTL to islet antigen peptides have not been identified. We used autologous blood monocyte-derived dendritic cells to elicit HLA-A2-restricted CTL to a peptide, MVWES-GCTV (aa 797-805), that is contiguous with a dominant CD4 T-cell epitope in the islet antigen tyrosine phosphatase IA-2, IA-2 peptide-specific CTL activity measured as Cr-51 release from autologous lymphoblasts was detected in 2/6 islet antibody-positive relatives at high risk for type 1 diabetes but also in 2/6 closely HLA-matched controls. All subjects had CTL activity to an HLA-A2-restricted Epstein-Barr virus peptide. CTL to the IA-2 self-peptide were therefore not disease-specific, consistent with other evidence that autoreactive T cells are present in healthy individuals. (C) 2001 Academic Press.
- Publisher
- ACADEMIC PRESS INC
- Keywords
- DIABETES-MELLITUS; DENDRITIC CELL; HUMAN BLOOD; PREDICTION; PANCREAS; ANTIGENS
- Publisher's Version
- https://doi.org/10.1006/clim.2001.5031
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-06-01 12:00:00