Development of dendritic cells in culture from human and murine thymic precursor cells
- Author(s)
- Kelly, KA; Lucas, K; Hochrein, H; Metcalf, D; Wu, L; Shortman, K;
- Details
- Publication Year 2001-02-01,Volume 47,Issue #1,Page 43-54
- Journal Title
- CELLULAR AND MOLECULAR BIOLOGY
- Publication Type
- Journal Article
- Abstract
- The earliest T-precursor population in the adult murine thymus can give rise to dendritic cells (DC) in culture if stimulated with a cocktail of cytokines that includes interleukin (IL)-3, but not with cytokine mixes based on granulocyte-macrophage colony stimulating factor (GM-CSF), normally used to generate myeloid-derived DC. This and other evidence led to the proposal that two different lineages of DC exist, one lymphoid-related and the other myeloid-related. To determine whether this selective response to cytokines was restricted to murine DC, early human thymic T-precursors were isolated and their capacity to generate DC in response to various cytokines directly compared to their murine counterparts. In contrast to cultures of murine thymic precursors, CD34(+)CDla(-) lineage marker negative (Lin(-)) precursor cells from the human thymus proliferated and generated DC with both the IL-3-containing cytokine mix lacking GM-CSF and with GM-CSF based cytokine mixes. These CD34(+)CDla(-) Lin- human precursor cells also gave rise to NK cells under appropriate culture conditions, but produced no granulocyte, monocyte, eosinophil, megakaryocyte or erythroid cells in standard soft-agar colony-forming cell assays. Thus, although apparently lymphoid-restricted, the human thymic DC precursors responded to the myeloid factor GM-CSF as well as to the cytokines selective for murine lymphoid-related DC.
- Publisher
- CELLULAR & MOLECULAR BIOLOGY
- Keywords
- COLONY-STIMULATING-FACTOR; NECROSIS-FACTOR-ALPHA; CD34(+) HEMATOPOIETIC PROGENITORS; CORD-BLOOD DIFFERENTIATE; BONE-MARROW PROGENITORS; C-KIT-LIGAND; NATURAL-KILLER; MONOCLONAL-ANTIBODIES; HUMAN-T; CD8 EXPRESSION
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Creation Date: 2001-02-01 12:00:00