Effect of granulocyte-macrophage colony-stimulating factor on the generation of epidermal Langerhans cells
- Author(s)
- Burnham, K; Robb, L; Scott, CL; O'Keeffe, M; Shortman, K;
- Details
- Publication Year 2000-12,Volume 20,Issue #12,Page 1071-1076
- Journal Title
- JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
- Publication Type
- Journal Article
- Abstract
- The role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and Flt3 ligand in the in vivo development of Langerhans cells (LC) was assessed, considering both the steady-state levels of LC in the epidermis and the rate of LC recovery after depletion following lipopolysaccharide (LPS) treatment. The density of LC was determined by counting following IA-specific immunofluorescent staining of epidermal sections from mouse ears, LC levels were compared in beta common chain receptor null (betac(-/-)) mice that fail to respond to GM-CSF interleukin-5 (IL-5), in GM-CSF transgenic mice with elevated GM-CSF levels, and in mice given daily injections of Flt3 ligand. In the steady state, LC levels were increased in GM-CSF transgenic mice and present at reduced levels in betac(-/-) mice but unchanged in Flt3 ligand-injected mice. Application of LPS to the ears of control BL/6 mice led to an approximately 70% reduction in LC 4 days later, with recovery beginning by day 8 and a return to normal levels by 2 weeks. This recovery was significantly delayed in betac(-/-) mice and unchanged in Flt3 ligand-injected mice. These results suggest that GM-CSF (but not Flt3 ligand) enhances recruitment/maturation of LC even though GM-CSF is not essential for their formation.
- Publisher
- MARY ANN LIEBERT INC PUBL
- Keywords
- DENDRITIC CELLS; NONLYMPHOID TISSUES; GM-CSF; MICE; LIPOPOLYSACCHARIDE; NUMBERS; SKIN; GROWTH; LIGAND
- Publisher's Version
- https://doi.org/10.1089/107999000750053735
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-12-01 12:00:00