The murine gene, Traube, is essential for the growth of preimplantation embryos
- Details
- Publication Year 2000-11-15,Volume 227,Issue #2,Page 324-342
- Journal Title
- DEVELOPMENTAL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- Little is known about the genetic control of preimplantation development. We have isolated, characterized, and mutated a previously undescribed mouse gene, Traube (Trb), essential for preimplantation development. Similar protein coding sequences are found in rats, humans, and yeast. The TRB protein contained two amino-terminal acidic domains, a leucine zipper, and three putative nuclear localization signals. The Trb gene was expressed at low levels ubiquitously early in development and became restricted to the liver and the central nervous system from E11.5 onward. Myc-tagged TRB protein was localized to the nucleus, and in a large proportion of the cells to the nucleoli. The Trb mutant embryos halted in development at the compacted morula stage at E2.5. At E3.5 they started to decompact and a day later they disintegrated and died. The observed defect was cell autonomous, as mutant cells failed to participate in the formation of chimeric embryos. The Trb mutant embryos showed a 50% reduction of the total cell number. The mutant embryos exhibited a paucity of ribosomes, polyribosomes, and rough endoplasmic reticulum. This paucity of ribosomes together with the localization of TRB to the nucleoli, the site of ribosome synthesis, suggests that TRB is involved in the synthesis of ribosomes. (C) 2000 Academic Press.
- Publisher
- ACADEMIC PRESS INC
- Keywords
- MOUSE DEVELOPMENT; CELL-ADHESION; SERINE/THREONINE KINASE; MAMMALIAN DEVELOPMENT; TARGETED DISRUPTION; FACTOR RECEPTORS; TRAP MUTATION; PROTEIN; EXPRESSION; CHROMATIN
- Publisher's Version
- https://doi.org/10.1006/dbio.2000.9915
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- Refer to copyright notice on published article.
Creation Date: 2000-11-15 12:00:00