Predominant transgene expression in exocrine pancreas directed by the CMV promoter
- Author(s)
- Zhan, Y; Brady, JL; Johnston, AM; Lew, AM;
- Details
- Publication Year 2000-11,Volume 19,Issue #11,Page 639-645
- Journal Title
- DNA AND CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- The enhancer/promoter of the human cytomegalovirus gene encoding the major immediate-early protein (CMVp) is reputed to be one of the strongest and most promiscuous regulatory elements for directing transcription of heterologous genes in vitro. However, transgene expression under the promoter in adult transgenic mice is often more restricted. We selected a CMVp segment from position -350 to +59 to control expression of transgenes for two secretory fusion proteins. Expression was analyzed by immunohistology staining and quantified by Northern blot, Western blot, and ELISA of secretions from explanted tissues. In all six lines of transgenic mice, the highest expression of transgenes at the mRNA and protein level was observed in the exocrine tissue of the pancreas, although the levels of expression varied among the lines. The results indicate not only that CMVp is not a universal promoter in vivo but indeed that it can be relatively specific for the exocrine pancreas, where expression of the gene it controlled was consistently very high.
- Publisher
- MARY ANN LIEBERT INC PUBL
- Keywords
- IMMEDIATE-EARLY PROMOTER; HUMAN CYTOMEGALOVIRUS; ENHANCER-PROMOTER; EARLY GENE; HYBRID GENE; CELL-LINE; IN-VIVO; MICE; MOUSE; PROTEIN
- Publisher's Version
- https://doi.org/10.1089/10445490050199045
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-11-01 12:00:00