Characterization of the EphA1 receptor tyrosine kinase: Expression in epithelial tissues
- Author(s)
- Coulthard, MG; Lickliter, JD; Subanesan, N; Chen, K; Webb, GC; Lowry, AJ; Koblar, S; Bottema, CDK; Boyd, AW;
- Details
- Publication Year 2001,Volume 18,Issue #4,Page 303-+
- Journal Title
- GROWTH FACTORS
- Publication Type
- Journal Article
- Abstract
- The Eph family (of receptor tyrosine kinases plays a crucial role during development and is implicated in oncogenesis. Using a partial cDNA clone of an Eph-related kinase (Esk) we isolated the complete coding region of a gene which we show to be murine EphA1 by both structural and functional criteria. The chromosomal localization is shown to be syntenic to hEphA1 and the genomic organization also shows distinct features found in the hEphA1 gene. Functionally, in keeping with findings for the human homologue, both soluble recombinant and "native" mEphA1 show preferential binding to ephrin A1. However, we also observed significant binding to other A-type ligands as has been observed for other Eph receptors. We analysed the expression of mEphA1 mRNA by in situ hybridization on tissue sections. mEphA1 was expressed in epithelial elements of skin, adult thymus, kidney and adrenal cortex. Taken together with previous Northern blotting data these results suggest that mEphA1 is expressed widely in differentiated epithelial cells.
- Publisher
- HARWOOD ACAD PUBL GMBH
- Keywords
- LIGAND-BINDING; CELL-LINES; AUTOPHOSPHORYLATION SITE; MELANOMA PROGRESSION; CRYSTAL-STRUCTURE; UP-REGULATION; GENE; FAMILY; DOMAIN; EPHRINS
- Publisher's Version
- https://doi.org/10.3109/08977190109029118
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-01-01 12:00:00