Fluorescence photobleaching analysis for the study of cellular dynamics

Author(s): Klonis, N; Rug, M; Harper, I; Wickham, M; Cowman, A; Tilley, L;
Details: Publication Year 2002-03,Volume 31,Issue #1,Page 36-51
Journal Title: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Publication Type: Journal Article
Abstract: The wide availability of the confocal microscope and the emergence of green fluorescent protein (GFP) transfection technology has led to the increasing use of photobleaching studies to examine aspects of cellular dynamics in living cells. In this review, we examine the theory and practice of performing photobleaching studies using a confocal microscope. We illustrate the application of photobleaching protocols using our own measurements of fluorescently labelled red blood cells and of malaria parasite-infected erythrocytes expressing GFP fusions and examine other examples from the literature.
Publisher: SPRINGER-VERLAG
Keywords: INTERNAL-REFLECTION-FLUORESCENCE; INTERMEDIATE FILAMENT NETWORKS; PLASMA-MEMBRANE PROTEINS; SPATIAL FOURIER-ANALYSIS; LATERAL DIFFUSION; TRANSLATIONAL DIFFUSION; ENDOPLASMIC-RETICULUM; LIVING CELLS; ROTATIONAL DIFFUSION; ERYTHROCYTE-MEMBRANE
Publisher's Version: https://doi.org/10.1007/s00249-001-0202-2
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2002-03-01 12:00:00