Progression of armed CTL from draining lymph node to spleen shortly after localized infection with herpes simplex virus 1
- Author(s)
- Coles, RM; Mueller, SN; Heath, WR; Carbone, FR; Brooks, AG;
- Details
- Publication Year 2002-01-15,Volume 168,Issue #2,Page 834-838
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- We have examined the generation of CTL immunity immediately after localized footpad infection with herpes simplex virus 1 (HSV-1) using three coordinated in vivo T cell tracking methodologies. Tetrameric MHC class I containing the immunodominant peptide from HSV-1 glycoprotein B (gB) showed that after infection the proportion of Ag-specific T cells peaked at day 5 within draining popliteal lymph nodes and 2 days later in the spleen. Preferential expression of the activation marker CD25 by tetramer-positive cells in draining popliteal nodes but not spleen suggested that gB-specific T cells were initially activated within the lymph node. In vivo cytotoxicity assays showed that Ag-specific effector cells were present within the draining lymph nodes as early as day 2 after infection, with a further 2-day lag before detection in the spleen. Consistent with the very early arming of effector CTL in the draining lymph node, adoptive transfer of CFSE-labeled gB-specific transgenic T cells showed that they had undergone one to four rounds of cell division by day 2 after infection. In contrast, proliferating T cells were first detected in appreciable numbers in the spleen on day 4, at which time they had undergone extensive cell division. These data demonstrate that HSV-1-specific T cells are rapidly activated and armed within draining lymph nodes shortly after localized HSV-1 infection. This is followed by their dissemination to other compartments such as the spleen, where they further proliferate in an Ag-independent fashion.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- NERVOUS-SYSTEM; T-LYMPHOCYTES; TYPE-1 INFECTION; C57BL/6 MICE; ANTIGEN; MEMORY; CELLS; DIFFERENTIATION; DIVISION; TOLERANCE
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- Refer to copyright notice on published article.
Creation Date: 2002-01-15 12:00:00