Generating mouse models of retinal disease using ENU mutagenesis
Details
Publication Year 2002-02, Volume 42, Issue #4, Page 479-485
Journal Title
VISION RESEARCH
Publication Type
Journal Article
Abstract
We used the chemical mutagen, N-ethyl-N-nitrosourea, to induce random point mutations in the germline of the mouse strain C57BL/6 in order to generate models of retinal diseases. 1163 mutagenised first generation mice produced using this approach were examined for eve abnormalities. Approximately one-third (412) presented with some form of ocular abnormality. Most changes were unilateral and confined to the anterior segment of the eve. Less than 10% (44) of identified changes affected the posterior segment of the eye. 21 mice with varying ocular abnormalities, including 17 with retinal changes, were bred to produce second generation mice to confirm genetic inheritance. Genetic inheritance was confirmed in several of these lines including three with retinal changes. (C) 2002 Elsevier Science Ltd. All rights reserved.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
ETHYLNITROSOUREA MUTAGENESIS; GENE; SPERMATOGONIA; DEGENERATION; MUTATIONS; MUTANTS
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2002-02-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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