The cross-priming APC requires a Rel-dependent signal to induce CTL
- Author(s)
- Mintern, JD; Belz, G; Gerondakis, S; Carbone, FR; Heath, WR;
- Details
- Publication Year 2002-04-01,Volume 168,Issue #7,Page 3283-3287
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Induction of OVA-specific CTL by cross-priming requires help from CD4 T cells, which use CD154 to signal CD40 on the APC. To further dissect the molecular pathways involved in cross-priming, we examined the role of Rel, an NF-kappaB family member. c-rel(-/-) mice failed to generate OVA-specific CTL by cross-priming, but could induce CTL to HSV-1. Using chimeric mice, Rel expression was shown to be required by the APC, but not by the T cells. Notably, the deficiency in Rel could be overcome by triggering CD40, implying that the APC required Rel before receipt of the CD40 signal. These data suggest that the cross-priming APC must receive two signals before it can stimulate CTL. The first signal is Rel dependent and is required before activation of CD4 helper T cells, which then deliver the second signal using CD154 to trigger CD40.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- CYTOTOXIC T-CELLS; FACTOR-KAPPA-B; IMMUNE-RESPONSES; IN-VIVO; LYMPHOCYTE-PROLIFERATION; EXHIBIT DEFECTS; DENDRITIC CELL; SELF-ANTIGENS; MICE LACKING; C-REL
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Creation Date: 2002-04-01 12:00:00