A single buffer that universally serves both restriction digestion and loading
- Author(s)
- Corbett, AJ; Brady, JL; Lew, AM;
- Details
- Publication Year 2002-05,Volume 21,Issue #1,Page 51-55
- Journal Title
- MOLECULAR BIOTECHNOLOGY
- Publication Type
- Journal Article
- Abstract
- Restriction digestion is routinely performed in a buffer compatible with the restriction enzyme used. To load the samples on agarose gels for electrophoresis it is then necessary to add a loading buffer. A 10X loading buffer is often used, and consists of a dye to track the electrophoresis and a dense solution so that the digestion mixture sinks into the well. We describe a new buffer, which acts as a universal digestion buffer as well as a loading, buffer. This avoids double handling of samples, which wastes both time and consumables. Importantly, the efficiency of digestion was found not to be significantly decreased in this new buffer.
- Publisher
- HUMANA PRESS INC
- Keywords
- ENDONUCLEASES
- Publisher's Version
- https://doi.org/10.1385/MB:21:1:051
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2002-05-01 12:00:00