Characterization of two TCR transgenic mouse lines specific for herpes simplex virus
- Author(s)
- Mueller, SN; Heath, WR; McLain, JD; Carbone, FR; Jones, CM;
- Details
- Publication Year 2002-04,Volume 80,Issue #2,Page 156-163
- Journal Title
- IMMUNOLOGY AND CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- To better understand the T cell-mediated processes involved in the immune response to herpes simplex virus type 1 (HSV-1) infection, two HSV-specific T cell receptor (TCR) transgenic mouse lines were produced. These mice (gBT-I.1 and gBT-I.3) are MHC class I-restricted and specific for the immunodominant peptide from HSV glycoprotein B (gB), gB(498-505) . Although derived from the same clone, the mice differ in the chromosomal location of the TCR transgenes and show marked differences in TCR alpha/beta expression on both CD4(+) and CD8(+) cells in the thymus. Despite this, peripheral CD8(+) T cells from both mice express equally high levels of the transgenic TCR and bind the K-b gB(498-505) tetramer to the same degree. In concordance with this, both were shown to respond equally well in vitro upon stimulation with the gB(498-505) peptide or HSV-infected cells. These data show that selection of broadly equivalent peripheral T-cell subsets can occur in the presence of distinctly different thymic T-cell subsets.
- Publisher
- BLACKWELL PUBLISHING ASIA
- Keywords
- CELL ANTIGEN RECEPTOR; T-LYMPHOCYTE RESPONSE; CD8 CORECEPTOR; GLYCOPROTEIN-B; ALPHA; MICE; EXPRESSION; INFECTION; SELECTION; REGION
- Publisher's Version
- https://doi.org/10.1046/j.1440-1711.2002.01071.x
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2002-04-01 12:00:00