The combined absence of NF-kappa B1 and c-Rel reveals that overlapping roles for these transcription factors in the B cell lineage are restricted to the activation and function of mature cells

Pohl, T; Gugasyan, R; Grumont, RJ; Strasser, A; Metcalf, D; Tarlinton, D; Sha, W; Baltimore, D; Gerondakis, S

Author(s): Pohl, T; Gugasyan, R; Grumont, RJ; Strasser, A; Metcalf, D; Tarlinton, D; Sha, W; Baltimore, D; Gerondakis, S;
Details: Publication Year 2002-04-02,Volume 99,Issue #7,Page 4514-4519
Journal Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type: Journal Article
Abstract: Transcription factors NF-KB1 and c-Rel, individually dispensable during embryogenesis, serve similar, yet distinct, roles in the function of mature hemopoietic cells. Redundancy among Rel/ NF-KB family members prompted an examination of the combined roles of c-Rel and NF-KB1 by using mice that lack both proteins. Embryonic development and the maturation of hemopoietic progenitors were unaffected in nfkb1(-/-)c-rel(-/-) mice. Peripheral T cell populations developed normally, but follicular, marginal zone, and CD5(+) peritoneal B cell populations all were reduced. In culture, a failure of mitogen-stimulated nfkb1(-/-)c-rel(-/-) B cells to proliferate was caused by a cell cycle defect in early G(1) that prevented growth. In vivo, defects in humoral immunity and splenic architecture seen in nfkbl(-/-) and c-rel(-/-) mice were exacerbated in the double mutant mice. These findings demonstrate that in the B lineage overlapping roles for NF-K81 and c-Rel appear to be restricted to regulating the activation and function of mature cells.
Publisher: NATL ACAD SCIENCES
Keywords: NF-KAPPA-B; LYMPHOCYTE-ACTIVATION; SUBUNIT COMPOSITION; IMMUNE-RESPONSES; EXHIBIT DEFECTS; B-1 CELLS; DIFFERENTIATION; EXPRESSION; PROTEINS; MICE
Publisher's Version: https://doi.org/10.1073/pnas.072071599
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2002-04-02 12:00:00