Analysis of candidate antagonists of IAP-mediated caspase inhibition using yeast reconstituted with the mammalian Apaf-1-activated apoptosis mechanism
- Author(s)
- Hawkins, CJ; Silke, J; Verhagen, AM; Foster, R; Ekert, PG; Ashley, DM;
- Details
- Publication Year 2001-10,Volume 6,Issue #5,Page 331-338
- Journal Title
- APOPTOSIS
- Publication Type
- Journal Article
- Abstract
- We have reconstituted the Apaf-1-activated apoptosis mechanism in Sacchromyces cerevisiae such that the presence of a constitutively active form of Apaf-1 together with both Caspase-9 and Caspase-3 results in yeast death. This system is a good model of the Apaf-1-activated pathway in mammalian cells: MIHA (XIAP/hILP), and to a lesser degree MIHB (c-IAP1/HIAP2) and MIHC (c-IAP-2/HIAP1) can inhibit caspases in this system, and protection by IAPs (inhibitor of apoptosis) can be abrogated by coexpression of the Drosophila pro-apoptotic proteins HID and GRIM or the mammalian protein DIABLO/Smac. Using this system we demonstrate that unlike DIABLO/Smac, other proteins which interact with mammalian IAPs (TAB-1, Zap-1, Traf-1 and Traf-2) do not act to antagonise IAP- mediated caspase inhibition.
- Publisher
- KLUWER ACADEMIC PUBL
- Keywords
- NECROSIS-FACTOR RECEPTOR; SACCHAROMYCES-CEREVISIAE; CELL-DEATH; SCHIZOSACCHAROMYCES-POMBE; DROSOPHILA REAPER; FAMILY PROTEINS; GRIM; HID; PROTEASE; SYSTEM
- Publisher's Version
- https://doi.org/10.1023/A:1011329917895
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-10-01 12:00:00