The role of antigen in the localization of naive, acutely activated, and memory CD8(+) T cells to the lung during influenza pneumonia
- Author(s)
- Topham, DJ; Castrucci, MR; Wingo, FS; Belz, GT; Doherty, PC;
- Details
- Publication Year 2001-12-15,Volume 167,Issue #12,Page 6983-6990
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The role of Ag in the recruitment and localization of naive, acutely activated, and memory CD8(+) T cells to the lung during influenza infection was explored using TCR-transgenic (Tg) mice. Naive, Thy1.2(+)CD8(+) OT-I TCR-Tg cells were primed and recruited to the lung after transfer into congenic Thy1.1(+) recipients challenged with a genetically engineered influenza virus (influenza A/WSN/33 (WSN)-OVA(I)) containing the K-b restricted OVA(257-264) epitope (siinfekl) in the viral neuraminidase stalk. However, if the transferred animals were infected with a similar influenza virus that expressed an irrelevant Kb epitope (WSN-PEPII), no TCR-Tg T cells were detectable in the lung, although they were easily visible in the lymphoid organs. Conversely, there were substantial numbers of OT-I cells found in the lungs of WSN-PEPII-infected mice when the animals had been previously, or were concurrently, infected with a recombinant vaccinia virus expressing OVA. Similar results were obtained with nontransgenic populations of memory CD8(+) T cells reactive to a murine gamma -herpes virus-68 Ag. Interestingly, the primary host response to the immunodominant influenza nucleoprotein epitope was not affected by the presence of memory or recently activated OT-I T cells. Thus, although Ag is required to activate the T cells, the subsequent localization of T cells to the lung during a virus infection is a property of recently activated and memory T cells and is not necessarily driven by Ag in the lung.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- RESPIRATORY VIRUS-INFECTIONS; MHC CLASS-I; AIRWAY SMOOTH-MUSCLE; SENDAI-VIRUS; INFLAMMATORY PROCESS; VIRAL-INFECTION; LYMPHOCYTIC CHORIOMENINGITIS; LEUKOCYTE RECRUITMENT; BYSTANDER ACTIVATION; IMMUNE SUPPRESSION
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Creation Date: 2001-12-15 12:00:00