The antigenicity of the chicken anaemia virus protein VP3 (Apoptin)
- Author(s)
- Cunningham, SC; Lew, AM; Tannock, GA;
- Details
- Publication Year 2001-12-01,Volume 30,Issue #6,Page 613-619
- Journal Title
- AVIAN PATHOLOGY
- Publication Type
- Journal Article
- Abstract
- Chicken anaemia virus protein VP3 (Apoptin) was cloned and expressed as a recombinant protein and evaluated for its suitability as a serodiagnostic reagent. VP3 was expressed as a fusion protein either with glutathione S-transferase or with a six-histidine tag. Both recombinant proteins reacted specifically with anti-VP3 monoclonal antibodies and with serum from vaccinated chickens by Western blot and by enzyme-linked immunosorbent assay (ELISA). However, when testing sera from birds of different ages and genetic backgrounds, high non-specific reactions were evident and false positives were observed, especially in older birds. This suggests that VP3 is poorly immunogenic during infection and low antibody concentrations are masked by non-specific reactions. Thus, VP3 is not suitable for use as antigen in ELISAs.
- Publisher
- CARFAX PUBLISHING
- Keywords
- LINKED-IMMUNOSORBENT-ASSAY; GLUTATHIONE-S-TRANSFERASE; BURSAL DISEASE VIRUS; ANEMIA AGENT; MONOCLONAL-ANTIBODIES; RECOMBINANT PROTEINS; SERUM ANTIBODY; POLYMERIZATION; PURIFICATION; EXPOSURE
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-12-01 12:00:00