Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs

Robb, L; Tarrant, J; Groom, J; Ibrahim, M; Li, RL; Borobakas, B; Wright, MD

Author(s): Robb, L; Tarrant, J; Groom, J; Ibrahim, M; Li, RL; Borobakas, B; Wright, MD;
Details: Publication Year 2001-11-11,Volume 1522,Issue #1,Page 31-41
Journal Title: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Publication Type: Journal Article
Abstract: Previous analyses of the murine and human TSSC6 (also known as Phemx) proteins were not carried out using the full length sequence. Using 5'-RACE and cDNA library screening, we identified an additional 5' sequence for both the murine Tssc6 cDNA and its human homologue TSSC6. This novel sequence encodes a 5' exon encoding an in frame, upstream start codon, an N-terminal cytoplasmic domain and a transmembrane domain. The deduced, and now full length, murine and human TSSC6 proteins contained four hydrophobic regions together with other features characteristic of the tetraspanin superfamily. Computational analyses of the full length sequences show that TSSC6 is a genuine, albeit relatively divergent member of this superfamily. Using RNA from a number of mouse tissues, we identified seven splice variants of Tssc6. Splice variants of the human gene were also detected. Tssc6 expression was detected early in embryogenesis in primitive blood cells and was confined to haematopoietic organs in the adult mouse. Tssc6 expression was detected in many haematopoietic cell lines and was highest in cell lines of the erythroid lineage. (C) 2001 Elsevier Science B.V. All rights reserved.
Publisher: ELSEVIER SCIENCE BV
Keywords: TUMOR-ASSOCIATED ANTIGEN; EMBRYONIC STEM-CELLS; TRANSMEMBRANE-4 SUPERFAMILY; MONOCLONAL-ANTIBODIES; SCHISTOSOMA-MANSONI; TM4SF PROTEINS; LYMPHOCYTES-T; CLASS-I; CLONING; SURFACE
Publisher's Version: https://doi.org/10.1016/S0167-4781(01)00306-2
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2001-11-11 12:00:00