The LIM domain gene LMO4 inhibits differentiation of mammary epithelial cells in vitro and is overexpressed in breast cancer
- Author(s)
- Visvader, JE; Venter, D; Hahm, K; Santamaria, M; Sum, EYM; O'Reilly, L; White, D; Williams, R; Armes, J; Lindeman, GJ;
- Details
- Publication Year 2001-12-04,Volume 98,Issue #25,Page 14452-14457
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- LM04 belongs to a family of LIM-only transcriptional regulators, the first two members of which are oncoproteins in acute T cell leukemia. We have explored a role for LM04, initially described as a human breast tumor autoantigen, in developing mammary epithelium and breast oncogenesis. Lmo4 was expressed predominantly in the lobuloalveoli of the mammary gland during pregnancy. Consistent with a role in proliferation, forced expression of this gene inhibited differentiation of mammary epithelial cells. Overexpression of LM04 mRNA was observed in 5 of 10 human breast cancer cell lines. Moreover, in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LM04 in 56% of specimens. immunohistochemistry confirmed overexpression in a high percentage (62%) of tumors. These studies imply a role for LM04 in maintaining proliferation of mammary epithelium and suggest that deregulation of this gene may contribute to breast tumorigenesis.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- COMPARATIVE GENOMIC HYBRIDIZATION; COPY NUMBER; ERYTHROID DEVELOPMENT; HOMEODOMAIN PROTEINS; TRANSGENIC MICE; LEUKEMIA; TRANSLOCATIONS; IDENTIFICATION; AMPLIFICATION; EXPRESSION
- Publisher's Version
- https://doi.org/10.1073/pnas.251547698
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2001-12-04 12:00:00