PRIOR CHEMOTHERAPY DOES NOT PREVENT EFFECTIVE MOBILIZATION BY G-CSF OF PERIPHERAL-BLOOD PROGENITOR CELLS
- Author(s)
- DELUCA, E; Sheridan, WP; WATSON, D; Szer, J; Begley, CG;
- Details
- Publication Year 1992-11,Volume 66,Issue #5,Page 893-899
- Journal Title
- BRITISH JOURNAL OF CANCER
- Publication Type
- Journal Article
- Abstract
- In this study we demonstrate that the hemopoietic growth factor, G-CSF successfully mobilised progenitor cell populations into the peripheral blood in a population of patients despite intensive pretreatment with chemotherapy. Administration of G-CSF increased the numbers of peripheral blood progenitor cells (PBPC) by a median of 76-fold above basal levels. Maximal levels of PBPC were observed on days 5 and 6 after G-CSF treatment. In two patients a second cycle of G-CSF mobilised PBPC to levels comparable with those seen after the first cycle of G-CSF treatment. An earlier hemopoietic cell population (pre-CFC's) was also mobilised with levels increased up to 50-fold above basal levels. Using a standard mononuclear cell leukapheresis technique the PBPC were collected extremely efficiently (essentially 100%) and could be further successfully enriched by separation using a Ficoll gradient. For patients who underwent the optimal collection protocol (i.e. leukapheresis on days 5, 6 and 7) a total of 32 +/- 6 x 10(4) GM-CFC kg-1 were collected. The ability to mobilise PBPC using G-CSF alone and to successfully and efficiently harvest these cells has important implications for the future of transplantation and high dose chemotherapy procedures.
- Publisher
- CHURCHILL LIVINGSTONE
- Keywords
- COLONY-STIMULATING FACTOR; HEMATOPOIETIC STEM-CELLS; HUMAN-BONE-MARROW; AUTOLOGOUS TRANSPLANTATION; COLLECTION; CULTURE; LIGAND; MOUSE
- Publisher's Version
- https://doi.org/10.1038/bjc.1992.381
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-11-01 12:00:00