STRUCTURE AND EXPRESSION OF THE GM-CSF RECEPTOR ALPHA-CHAIN AND BETA-CHAIN GENES IN HUMAN LEUKEMIA
Details
Publication Year 1993-01, Volume 7, Issue #1, Page 63-74
Journal Title
LEUKEMIA
Publication Type
Journal Article
Abstract
In this paper we report on the structure and expression of the genes encoding the alpha and beta chains of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor in human leukemia. The alpha chain gene is highly polymorphic in normal individuals and no evidence for rearrangement within this locus was found in 47 hemopoietic, nine non-hemopoietic malignancies and five human cell lines. Using the polymerase chain reaction the gene for the alpha chain was shown to be expressed in 18/18 primary myeloid as well as 8/8 primary lymphoid leukemias analysed. To investigate the integrity of the mRNA, polymerase chain reactions (PCR) using a combination of oligonucleotides spanning the entire coding region of the alpha chain were performed. Normal sized fragments were generated with all combinations of oligonucleotides from all but one leukemia. One chronic lymphoid leukemia displayed an apparent alteration at the 3' end of the 3' untranslated region of the alpha chain mRNA. No polymorphisms were detected in the beta chain gene which was also not rearranged in any of the samples analysed. The beta chain mRNA was expressed in 17/18 primary myeloid and 7/8 primary lymphoid leukemias and in those leukemias there was no evidence for any lesions in the mRNA, as judged by PCR fragment size. Thus gross structural lesions in the genes encoding the GM-CSF receptor alpha and beta chains appear to be infrequent in hemopoietic neoplasms.
Publisher
STOCKTON PRESS
Keywords
COLONY-STIMULATING FACTOR; ACUTE MYELOBLASTIC-LEUKEMIA; GROWTH-FACTOR RESPONSIVENESS; CELL-LINES; ERYTHROPOIETIN RECEPTOR; MYELOID-LEUKEMIA; HEMATOPOIETIC-CELLS; MOLECULAR-CLONING; INHIBITORY FACTOR; POINT MUTATION
Rights Notice
Refer to copyright notice on published article.


Creation Date: 1993-01-01 12:00:00
Last Modified: 0001-01-01 12:00:00
An error has occurred. This application may no longer respond until reloaded. Reload 🗙