MARROW PROLIFERATION AND THE APPEARANCE OF GIANT NEUTROPHILS IN RESPONSE TO RECOMBINANT HUMAN GRANULOCYTE COLONY STIMULATING FACTOR (RHG-CSF)
- Author(s)
- Campbell, LJ; Maher, DW; TAY, DLM; Boyd, AW; Rockman, S; McGrath, K; Fox, RM; MORSTYN, G;
- Details
- Publication Year 1992-03,Volume 80,Issue #3,Page 298-304
- Journal Title
- BRITISH JOURNAL OF HAEMATOLOGY
- Publication Type
- Journal Article
- Abstract
- During a study of recombinant human granulocyte colony stimulating factor (rhG-CSF) administration, 15 patients received twice daily i.v. infusions and nine patients received daily s.c. infusions of rhG-CSF for 5 d prior to cytotoxic therapy, and then a second course subsequent to melphalan administration. There was a striking dose-related neutrophilia and the appearance in the blood of early myeloid cells that express the intercellular adhesion molecule CD54. In addition, giant neutrophils or macropolycytes were observed in the peripheral blood of all patients. These cells were evident on the display of the Technicon H*1 as a population of large peroxidase positive cells, and using Feulgen staining these cells were shown to be tetraploid. Bone marrow kinetics studies performed on Day 4 or 5 indicated an increase in the proportion of bone marrow cells in S phase, G2 and mitosis, reflecting a proliferative response of the marrow. Large myeloid precursors and occasional binucleate promyelocytes were seen in the bone marrows done on Days 14 and 18 but not on Day 5. These findings indicate that administered G-CSF has both quantitative and qualitative effects on myeloid cells in vivo.
- Publisher
- BLACKWELL SCIENCE LTD
- Keywords
- TRANSITIONAL-CELL-CARCINOMA; NEUTROPENIA; CHEMOTHERAPY; EXPRESSION; UROTHELIUM; ADHESION; THERAPY
- Publisher's Version
- https://doi.org/10.1111/j.1365-2141.1992.tb08136.x
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-03-01 12:00:00