SYNERGISTIC SUPPRESSION - ANOMALOUS INHIBITION OF THE PROLIFERATION OF FACTOR-DEPENDENT HEMATOPOIETIC-CELLS BY COMBINATION OF 2 COLONY-STIMULATING FACTORS
- Author(s)
- Metcalf, D; Nicola, NA; Gough, NM; ELLIOTT, M; McArthur, G; Li, M;
- Details
- Publication Year 1992-04-01,Volume 89,Issue #7,Page 2819-2823
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Cells of the continuous murine hemopoietic cell line FDC-P1 expressing macrophage-colony-stimulating factor (M-CSF) receptors following retroviral insertion of murine c-fms cDNA proliferated clonally when stimulated by granulocyte/macrophage (GM)-CSF, multipotential CSF, or M-CSF. However, M-CSF combined with either GM-CSF or multi-CSF, even at low CSF concentrations, strongly inhibited colony formation, with loss of clonogenicity in affected cells accompanied by increased macrophage differentiation. Stimulation by these CSF combinations did not induce short-term changes in CSF receptor expression or internalization. FDC-P1 cells expressing another inserted tyrosine kinase receptor, basic fibroblast growth factor receptor, did not exhibit suppression when GM-CSF was combined with fibroblast growth factor. This phenomenon of synergistic suppression may have relevance for the future clinical use of combinations of CSFs, because a potentially similar suppression is also observable with some normal macrophage progenitor cells.
- Publisher
- NATL ACAD PRESS
- Keywords
- GM-CSF; GROWTH-FACTOR; DIFFERENTIATION; RECEPTOR
- Publisher's Version
- https://doi.org/10.1073/pnas.89.7.2819
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-04-01 12:00:00